Digital Book
Microbial Electrochemical Cell Technology with Lab Notes
Chapter 6 - Biohydrogen Production in Microbial Electrolysis Cells
Author: Prof. Dr. Tunc Catal
Publication Date: 10.01.2026
Publisher: EUCHEMBIOJ Publishing Platform
DOI:
10.62063/2026-mfc-c6
Type:
Applied Handbook
Field:
Biotechnology, Bioelectrochemistry
ABSTRACT
An overview of microbial electrolysis cells (MECs) is given in this chapter, with particular attention to their advantages over tradition al microbial fuel cells, system components, and operating principles. Microorganisms at the cathode of MECs are able to transform organ ic substrates into hydrogen or other useful chemicals by means of an external voltage. The chapter covers the effects of electrode materials, microbial community composition, and operational parameters, especially applied voltage, on energy efficiency and hydrogen yield. Important issues like material costs, cathode overpotentials, and re actor design constraints are also discussed. With the potential to be integrated into future energy and waste management systems, MECs are highlighted as promising technologies for sustainable hydrogen production using wastewater and renewable resources.
Keywords
Microbial electrolysis cells; Biohydrogen production; Applied voltage; Electron transfer mechanisms; Wastewater valorization
References
Aelterman, P., Rabaey, K., Pham, T. H., Clauwaert, P., & Verstraete, W. (2006). Continuous electricity generation from organic pollutants by bioelectrochemical reactors. Environmental Science & Technology, 40(11), 3388-3394.
Logan, B. E., Cheng, S., Ding, S. J., Kim, S., Li, B., Oh, S. E., … & регион, П. (2006). Microbial electrolysis cells for hydrogen production from wastewater. Environmental Science & Technology, 40(17), 5181-5185.
Rozendal, R. A., Hamelers, H. V. M., Molenaar, R. A., Buisman, C. J. N. (2006). Towards practical implementation of bioelectrochemical wastewater treatment. Trends in Biotechnology, 24(11), 491-499.
Rabaey, K., Angenent, L. T., Schroder, U., & Keller, J. (2007). Bioelectrochemical systems: from microbial fuel cells to microbial electrolysis cells. Water Science & Technology, 56(1), 11-19.
Escapa, A., Mateos, R., Diaz-Marcos, J., & Morán, A. (2018). Microbial electrolysis cells: A review on fundamentals, technology and applications. Energy Conversion and Management, 157, 139-159.
Jeremiasse, A. W., Hamelers, H. V. M., & Buisman, C. J. N. (2010). Energy balance of microbial electrolysis cells for hydrogen production from acetate. Environmental Science & Technology, 44(15), 5793-5799.
Li, B., Cheng, S., & Logan, B. E. (2008). Electricity generation by thermophilic microbial fuel cells. Applied Microbiology and Biotechnology, 79(3), 493-499.
Pant, D., Van Bogaert, G., Diels, L., & Vanbroekhoven, K. (2010). A review of the substrates used in microbial fuel cells (MFCs) for sustainable energy production. Bioresource Technology, 101(23), 8501-8518.
Wagner, J., Harnisch, F., Schroder, U., & Rosenbaum, M. (2009). Scale-up of microbial electrolysis cells for hydrogen production. Bioresource Technology, 100(22), 4946-4951.
Zhao, Z. W., Jiang, Y., Zhang, Y. P., Li, X. Y., & Li, J. S. (2016). A review of electrode materials for microbial electrolysis cells. International Journal of Hydrogen Energy, 41(36), 16227-16240.
Logan, B. E., Cheng, S., Watson, D. F., Estadt, G., Rozendal, R. A., & Jerke, J. W. (2007). Microbial electrolysis cells for hydrogen production from wastewater. Environmental Science & Technology Letters, 41(17), 5181-5185.
Lovley, D. R. (2006). Bug juice: electricity generation from wastes using microbial fuel cells. Environmental Microbiology, 8(6), 944-956.
Rabaey, K., & Rozendal, R. A. (2010). Microbial electrolysis cells: novel biotechnology for energy production from organic wastes. Trends in Biotechnology, 28(8), 446-453.
Schröder, U., Harnisch, F., Angenent, L., & Holtmann, D. (2003). Microbial electron transfer beyond the cell surface. Angewandte Chemie International Edition, 42(25), 2880-2883.
Rabaey, K., Angenent, L. T., Schröder, U., & Keller, J. (2007). Bioelectrochemical systems: From microbial fuel cells to microbial electrolysis cells. Water Science and Technology, 56(1), 11-19.
Logan, B. E., Cheng, S., Ding, S. J., Regan, J. M., Aelterman, P., Aelterman, P., Clauwaert, P., & Rabaey, K. (2006). Microbial electrolysis cells for hydrogen production. Environmental Science & Technology Letters, 40(17), 5181-5185.
Rozendal, R. A., Hamelers, H. V. M., & Buisman, C. J. N. (2006). Effects of anode potential on direct electron transfer from wastewater bacteria to graphite electrodes. Applied and Environmental Microbiology, 72(4), 2324-2329.
Freguia, S., Logan, B. E., Rabaey, K., & Park, H. D. (2008). Electrohydrogenesis in microbial electrolysis cells (MECs). Water Research, 42(16), 4919-4928.
Liu, H., Grot, S., & Logan, B. E. (2005). Electrochemically assisted microbial production of hydrogen from acetate. Environmental Science & Technology Letters, 39(11), 4317-4320.
Escapa, A., Méndez, L., Mateo, R., & Morán, A. (2016). A review on microbial electrolysis cells (MECs): From fundamentals to future prospects. Renewable and Sustainable Energy Reviews, 55, 794-812.
Kumar, G.,жным, A., Kumar, S.,жным, R., &жным, M. (2021). Microbial electrolysis cells for biohydrogen production: A critical review. Bioresource Technology, 319, 124184.
Dong, H., Feng, C., Wang, F., Zhang, Y., Qu, F., Li, Y., & Ren, N. (2020). Recent advances in cathode materials for microbial electrolysis cells: Challenges and perspectives. Applied Catalysis B: Environmental, 264, 118502.
Lu, L., Ren, N., Zhao, B., Wang, H., & Xing, D. (2010). Biohydrogen production from cassava starch wastewater using a two-stage anaerobic digestion and microbial electrolysis cell system. International Journal of Hydrogen Energy, 35(12), 6270-6276.
Sleutels, T. H. J. A., Ter Heijne, A., Buisman, C. J. N., & Hamelers, H. V. M. (2009). Selective enrichment on electrodes reveals key bacterial species for electricity production from acetate in bioelectrochemical systems. Environmental Science & Technology Letters, 43(21), 8263-8269.
Abadikhah, M., Liu, M., Persson, F., Wilén, B. M., Farewell, A., Sun, J., & Modin, O. (2023). Effect of anode material and dispersal limitation on the performance and biofilm community in microbial electrolysis cells. Biofilm, 6, 100161. https://doi.org/10.1016/j.bioflm.2023.100161
Abd-Elrahman, N. K., Al-Harbi, N., Al-Hadeethi, Y., Alruqi, A. B., Mohammed, H., Umar, A., & Akbar, S. (2022). Influence of Nanomaterials and Other Factors on Biohydrogen Production Rates in Microbial Electrolysis Cells-A Review. Molecules (Basel, Switzerland), 27(23), 8594. https://doi.org/10.3390/molecules27238594
Akagunduz, D., Cebecioglu, R., Ozdemir, M., & Catal, T. (2021). Removal of psychoactive pharmaceuticals from wastewaters using microbial electrolysis cells producing hydrogen. Water Science and Technology, 84 (4), 931-940. https://doi.org/10.2166/wst.2021.269
Akagunduz, D., Aydin, O., Tuncay, E., & Bermek, H. (2025). Microbial fuel cells: A potent and sustainable solution for heavy metal removal. Euchembioj Reviews, 1, 45–69. https://doi.org/10.62063/rev-6
Alcaraz-Gonzalez, V., Rodriguez-Valenzuela, G., Gomez-Martinez, J. J., Dotto, G. L., & Flores-Estrella, R. A. (2021). Hydrogen production automatic control in continuous microbial electrolysis cells reactors used in wastewater treatment. Journal of environmental management, 281, 111869. https://doi.org/10.1016/j.jenvman.2020.111869
Amar-Dubrovin, I., Ouaknin Hirsch, L., Rozenfeld, S., Gandu, B., Menashe, O., Schechter, A., & Cahan, R. (2022). Hydrogen Production in Microbial Electrolysis Cells Based on Bacterial Anodes Encapsulated in a Small Bioreactor Platform. Microorganisms, 10(5), 1007. https://doi.org/10.3390/microorganisms10051007
Atasever-Arslan, B., Akdoğan, E., Çakmak-Cebeci, F., & Catal, T. (2020). Bioelectricity generation using human neuronal-like cells in single chamber biofuel cells. Journal of Cleaner Production, 271, 122505. https://doi.org/10.1016/j.jclepro.2020.122505
Badwal, S. P., Giddey, S. S., Munnings, C., Bhatt, A. I., & Hollenkamp, A. F. (2014). Emerging electrochemical energy conversion and storage technologies. Frontiers in chemistry, 2, 79. https://doi.org/10.3389/fchem.2014.00079
Bajracharya, R., & Logan, B. E. (2010). Effects of pH on microbial fuel cell performance. Bioresource Technology, 101(13), 5452-5457.
Bahari, M.B., Mamat, C.R., Jalil, A.A., Hassan, N.S., Sawal, M.H., Rajendran, S., Alam, M.N.H.Z. (2024). Molybdenum as Cathode Materials: Paving the Way for Sustainable Biohydrogen Production in Microbial Electrolysis Cells. Process Safety and Environmental Protection (in press).
Bard, A. J., & Faulkner, L. R. (2001). Electrochemical power sources: Fundamentals and applications. Wiley.
Borole, A.P., Reguera, G., Ringeisen, B., Wang, Z.W., Feng, Y., Kim, B.H. 2011. Electroactive biofilms: current status and future research needs. Energy Environ Sci, 4, 4813-4834. https://doi.org/10.1039/c1ee02511b
Bond, D.R., Lovley, D.R. (2005). Evidence for involvement of an electron shuttle in electricity generation by Geothrix fermentans. Appl Environ Microbiol, 71, 2186-2189. https://doi.org/10.1128/AEM.71.4.2186-2189.2005
Borja-Maldonado, F., & Zavala, M.A.L. (2022). Contribution of configurations, electrode and membrane materials, electron transfer mechanisms, and cost of components on the current and future development of microbial fuel cells. Heliyon, 8, e09849. https://doi.org/10.1016/j.heliyon.2022.e09849
Call, D.F., Merrill, M.D., & Logan, B.E. (2009). High surface area stainless steel brushes as cathodes in microbial electrolysis cells. Environmental science & technology, 43(6), 2179–2183. https://doi.org/10.1021/es803074x
Catal, T. (2024). Could hydrogen gas be produced using human cells?. Clean Energy, 8(4), 34–39, https://doi.org/10.1093/ce/zkae034
Catal, T., & Liu, H. (2025). Microbial fuel cell technology: Novelties for a clean future. Euchembioj Reviews, 1, 1–20. https://doi.org/10.62063/rev-1
Catal, T., Liu, H., Kilinc, B., & Yilancioglu, K. (2024). Extracellular polymeric substances in electroactive biofilms play a crucial role in improving the efficiency of microbial fuel and electrolysis cells. Letters in Applied Microbiology, 77, 3, ovae017, https://doi.org/10.1093/lambio/ovae017
Catal, T., Li, K., Bermek, H., & Liu, H. (2008a). Electricity production from twelve monosaccharides using microbial fuel cells. Journal of Power Sources, 175 (1), 196-200. https://doi.org/10.1016/j.jpowsour.2007.09.083
Catal, T., Xu, S., Li, K., Bermek, H., & Liu, H. (2008b). Electricity generation from polyalcohols in single-chamber microbial fuel cells. Biosensors & bioelectronics, 24(4), 855–860. https://doi.org/10.1016/j.bios.2008.07.015
Catal, T., Cysneiros, D., O’Flaherty, V., & Leech, D. (2011). Electricity generation in single-chamber microbial fuel cells using a carbon source sampled from anaerobic reactors utilizing grass silage. Bioresource technology, 102 (1), 404-410. https://doi.org/10.1016/j.biortech.2010.07.006
Catal, T., Lesnik, K. L., & Liu, H. (2015). Suppression of methanogenesis for hydrogen production in single-chamber microbial electrolysis cells using various antibiotics. Bioresource Technology, 187, 77-83. https://doi.org/10.1016/j.biortech.2015.03.099
Catal, T. (2016). Comparison of various carbohydrates for hydrogen production in microbial electrolysis cells. Biotechnology & Biotechnological Equipment, 30 (1), 75-80. https://doi.org/10.1080/13102818.2015.1081078
Catal, T., Gover, T., Yaman, B., Droguetti, J., & Yilancioglu, K. (2017). Hydrogen production profiles using furans in microbial electrolysis cells. World Journal of Microbiology and Biotechnology, 33, 1-6. https://doi.org/10.1007/s11274-017-2270-1
Catal, T., Liu, H., Fan, Y., & Bermek, H. (2019a). A clean technology to convert sucrose and lignocellulose in microbial electrochemical cells into electricity and hydrogen. Bioresource technology reports, 5, 331-334. https://doi.org/10.1016/j.biteb.2018.10.002
Catal, T., Kul, A., Atalay, V.E., Bermek, H., Ozilhan, S., & Tarhan, N. (2019b). Efficacy of microbial fuel cells for sensing of cocaine metabolites in urine-based wastewater. Journal of Power Sources, 414, 1-7. https://doi.org/10.1016/j.jpowsour.2018.12.078
Catal, T., Pasaoglu, E., Akagunduz, D., Cebecioglu, R., Akul, N.B., & Ozdemir, M. (2021). Enhanced hydrogen production by mevastatin in microbial electrolysis cells. International Journal of Energy Research, https://doi.org/10.1002/er.6669
Cebecioglu, R., Akagunduz, D., & Catal, T. (2021). Hydrogen production in single-chamber microbial electrolysis cells using Ponceau S dye. 3 Biotech, 11(1), 27. https://doi.org/10.1007/s13205-020-02563-0
Cheng, S., Xing, D., Call, D.F., & Logan, B.E. (2009). Direct biological conversion of electrical current into methane by electromethanogenesis. Environ Sci Technol, 43, 3953-3958. https://doi.org/10.1021/es803531g
Cheng, D., Ngo, H. H., Guo, W., Chang, S. W., Nguyen, D. D., Zhang, S., Deng, S., An, D., & Hoang, N. B. (2022). Impact factors and novel strategies for improving biohydrogen production in microbial electrolysis cells. Bioresource technology, 346, 126588. https://doi.org/10.1016/j.biortech.2021.126588
Chiranjeevi, P., & Patil, S. A. (2020). Strategies for improving the electroactivity and specific metabolic functionality of microorganisms for various microbial electrochemical technologies. Biotechnology advances, 39, 107468. https://doi.org/10.1016/j.biotechadv.2019.107468
Chung, T. H., Meshref, M. N. A., Hai, F. I., Al-Mamun, A., & Dhar, B. R. (2020). Microbial electrochemical systems for hydrogen peroxide synthesis: Critical review of process optimization, prospective environmental applications, and challenges. Bioresource technology, 313, 123727. https://doi.org/10.1016/j.biortech.2020.123727
Costa, N.L., Olorounto, G., Lebègue, E., & Barrière, F. (2022). Electrografted anthraquinone to monitor pH at the biofilm-anode interface in a wastewater microbial fuel cell. Colloids and Surfaces B: Biointerfaces, 210, 112274. https://doi.org/10.1016/j.colsurfb.2021.112274
Cui, W., Luo, H., & Liu, G. (2023). Efficient hydrogen production in single-chamber microbial electrolysis cell with a fermentable substrate under hyperalkaline conditions. Waste management (New York, N.Y.), 171, 173–183. Advance online publication. https://doi.org/10.1016/j.wasman.2023.08.017
Dai, K., Yan, Y., Wang, Q. T., Zheng, S. J., Huang, Z. Q., Sun, T., Zeng, R. J., & Zhang, F. (2022). Electricity production and key exoelectrogens in a mixed-culture psychrophilic microbial fuel cell at 4 °C. Applied microbiology and biotechnology, 106(12), 4801–4811. https://doi.org/10.1007/s00253-022-12042-6
Ding, Y.H., Hixcon K.K., Aklujkar, M.A., Lipton, M.S., Smith, R.D., Lovley, D.R., & Mester, T. (2008). Proteome of Geobacter sulfurreducens grown with Fe(III) oxide or Fe(III) citrate as the electron acceptor. Biochim Biophys Acta, 1784, 1935-1941. https://doi.org/10.1016/j.bbapap.2008.06.011
Dos Passos, V. F., Marcilio, R., Aquino-Neto, S., Santana, F. B., Dias, A. C. F., Andreote, F. D., de Andrade, A. R., & Reginatto, V. (2019). Hydrogen and electrical energy co-generation by a cooperative fermentation system comprising Clostridium and microbial fuel cell inoculated with port drainage sediment. Bioresource technology, 277, 94–103. https://doi.org/10.1016/j.biortech.2019.01.031
Dubrovin, I. A., Hirsch, L. O., Chiliveru, A., Jukanti, A., Rozenfeld, S., Schechter, A., & Cahan, R. (2024). Microbial Electrolysis Cells Based on a Bacterial Anode Encapsulated with a Dialysis Bag Including Graphite Particles. Microorganisms, 12(7), 1486. https://doi.org/10.3390/microorganisms12071486
Dumas, C., Basseguy, R., & Bergel, A. (2008). DSA to grow electrochemically active biofilms of Geobacter sulfurreducens. Electrochimica Acta, 53, 3200-3209. https://doi.org/10.1016/j.electacta.2007.10.066
El-naggar, M.Y., Wanger, G., Leung, K.M., Yuzvinsky, T.D., Southam, G., Yang, J., Lau, W.M., Nealson, K.H., & Gorby, Y.A. (2010). Electrical transport along bacterial nanowires from Shewanella oneidensis MR-1. Proc Natl Acad Sci USA, 19, 18127-31. https://doi.org/10.1073/pnas.1004880107
Escapa, A., San-Martín, M. I., Mateos, R., & Morán, A. (2015). Scaling-up of membraneless microbial electrolysis cells (MECs) for domestic wastewater treatment: Bottlenecks and limitations. Bioresource technology, 180, 72–78. https://doi.org/10.1016/j.biortech.2014.12.096
Escapa, A., Mateos, R., Martínez, E.J., Blanes, J. (2016). Microbial electrolysis cells: An emerging technology for wastewater treatment and energy recovery. From laboratory to pilot plant and beyond. Renewable and Sustainable Energy Reviews, 55, 942-956. https://doi.org/10.1016/j.rser.2015.11.029
Fan, Y., Xu, S., Schaller, R., Jiao, J., Chaplen, F., & Liu, H. (2011). Nanoparticle decorated anodes for enhanced current generation in microbial electrochemical cells. Biosensors & bioelectronics, 26(5), 1908–1912. https://doi.org/10.1016/j.bios.2010.05.006
Fan, Y., Janicek, A., & Liu, H. (2024). Stable and high voltage and power output of CEA-MFCs internally connected in series (iCiS-MFC). The European Chemistry and Biotechnology Journal, 1, 47–57. https://doi.org/10.62063/ecb-17
Flayac, C., Trably, E., & Bernet, N. (2018). Microbial anodic consortia fed with fermentable substrates in microbial electrolysis cells: Significance of microbial structures. Bioelectrochemistry (Amsterdam, Netherlands), 123, 219–226. https://doi.org/10.1016/j.bioelechem.2018.05.009
Ghasemi, B., Yaghmaei, S., Abdi, K., Mardanpour, M. M., & Haddadi, S. A. (2020). Introducing an affordable catalyst for biohydrogen production in microbial electrolysis cells. Journal of bioscience and bioengineering, 129(1), 67–76. https://doi.org/10.1016/j.jbiosc.2019.07.001
Gorby, Y.A., Yanina, S., Mclean, J.S., Rosso, K.M., Moyles, D., Dohnalkova, A., Beveridge, T.J., Chang, I.S., Kim, B.H., Kim, K.S., Culley, D.E., Reed, S.B., Romine, M.F., Saffarini, D.A., Hill, E.A., Shi, L., Elias, D.A., Kennadey, D.W., Pinchuk, G., Watanabe, K., Ishii, S., Logan, B., Nealson, K.H., & Frederickson, J.K. (2006). Electrically conductive bacterial nanowires produced by Shewanella oneidensis strain MR-1 and other microorganisms. Proc Natl Acad Sci USA, 103, 11358-63. https://doi.org/10.1073/pnas.0604517103
Guo, H., & Kim, Y. (2019). Stacked multi-electrode design of microbial electrolysis cells for rapid and low-sludge treatment of municipal wastewater. Biotechnology for biofuels, 12, 23. https://doi.org/10.1186/s13068-019-1368-0
He, K., Li, W., Tang, L., Li, W., Lv, S., & Xing, D. (2022). Suppressing Methane Production to Boost High-Purity Hydrogen Production in Microbial Electrolysis Cells. Environmental science & technology, 56(17), 11931–11951. https://doi.org/10.1021/acs.est.2c02371
Hu, H., Fan, Y., & Liu, H. (2008). Hydrogen production using single-chamber membrane-free microbial electrolysis cells. Water research, 42(15), 4172–4178. https://doi.org/10.1016/j.watres.2008.06.015
Hu, H., Fan, Y., & Liu, H. (2009). Hydrogen production in single-chamber tubular microbial electrolysis cells using non-precious-metal catalysts. International journal of hydrogen energy, 34, 8535-8542. https://doi.org/10.1016/j.ijhydene.2009.08.011
Izallalen, M., Mahadevan, R., Burgard, A., Postier, B., Didanato, R., Sun, J., Schilling, C.H., & Lovely, D.R. (2008). Geobacter sulfurreducens strain engineered for increased rates of respiration. Metab Eng, 10, 267-75. https://doi.org/10.1016/j.ymben.2008.06.005
Jeremiasse, A. W., Hamelers, H. V., Kleijn, J. M., & Buisman, C. J. (2009). Use of biocompatible buffers to reduce the concentration overpotential for hydrogen evolution. Environmental science & technology, 43(17), 6882–6887. https://doi.org/10.1021/es9008823
Ji, X., Liu, X., Yang, W., Xu, T., Wang, X., Zhang, X., Wang, L., Mao, X., & Wang, X. (2022). Sustainable phosphorus recovery from wastewater and fertilizer production in microbial electrolysis cells using the biochar-based cathode. The Science of the total environment, 807(Pt 2), 150881. https://doi.org/10.1016/j.scitotenv.2021.150881
Jitaru, M. (2007). Electrochemical carbon dioxide reduction-fundamental and applied topics. J Univ Chem Technol Metall, 42, 333-344.
Kaneko, M., Ishihara, K., & Nakanishi, S. (2020). Redox-Active Polymers Connecting Living Microbial Cells to an Extracellular Electrical Circuit. Small (Weinheim an der Bergstrasse, Germany), 16(34), e2001849. https://doi.org/10.1002/smll.202001849
Kas, A., & Yilmazel, Y. D. (2022). High current density via direct electron transfer by hyperthermophilic archaeon, Geoglobus acetivorans, in microbial electrolysis cells operated at 80 °C. Bioelectrochemistry (Amsterdam, Netherlands), 145, 108072. https://doi.org/10.1016/j.bioelechem.2022.108072
Kilinc, B., Akagunduz, D., Ozdemir, M., Kul, A. & Catal, T. (2023). Hydrogen production using cocaine metabolite in microbial electrolysis cells. 3 Biotech, 13, 382. https://doi.org/10.1007/s13205-023-03805-7
Kyazze, G., Popov, A., Dinsdale, R., Esteves, S., Hawkes, F., Premier, G., & Guwy, A. (2010). Influence of catholyte pH and temperature on hydrogen production from acetate using a two chamber concentric tubular microbial electrolysis cell. International Journal of Hydrogen Energy, 35, 7716-7722. https://doi.org/10.1016/j.ijhydene.2010.05.036
Lee, M. Y., Kim, K. Y., Yang, E., & Kim, I. S. (2015). Evaluation of hydrogen production and internal resistance in forward osmosis membrane integrated microbial electrolysis cells. Bioresource technology, 187, 106–112. https://doi.org/10.1016/j.biortech.2015.03.079
Li, W., Wang, Y., Li, Y., & Liu, H. (2021). Microbial electrolysis cells: A comprehensive review on recent developments and future prospects. Renewable Energy, 173, 1118-1136.
Logan, B.E. (2009). Exoelectrogenic bacteria that power microbial fuel cells. Nature Rev Microbiol, 7, 375-381. https://doi.org/10.1038/nrmicro2113
Logan, B. E., & Rabaey, K. (2012). Conversion of wastes into bioelectricity and chemicals by using microbial electrochemical technologies. Science (New York, N.Y.), 337(6095), 686–690. https://doi.org/10.1126/science.1217412
Lovley, D.E. (2011). Live wires: direct extracellular electron exchange for bioenergy and the bioremediation of energy-related contamination. Energy Environ Sci, 4, 4896-4906. https://doi.org/10.1039/c1ee02229f
Lusk, B. G., Colin, A., Parameswaran, P., Rittmann, B. E., & Torres, C. I. (2018). Simultaneous fermentation of cellulose and current production with an enriched mixed culture of thermophilic bacteria in a microbial electrolysis cell. Microbial biotechnology, 11(1), 63–73. https://doi.org/10.1111/1751-7915.12733
Magdalena, J. A., Pérez-Bernal, M. F., Bernet, N., & Trably, E. (2023). Sequential dark fermentation and microbial electrolysis cells for hydrogen production: Volatile fatty acids influence and energy considerations. Bioresource technology, 374, 128803. https://doi.org/10.1016/j.biortech.2023.128803
Marsili, E., Baron, D.B., Shikhare, I.D., Coursolle, D., Gralnick, J.A., & Bond, D.R. (2008). Shewanella secretes flavins that mediate extracellular electron transfer. Proc Natl Acad Sci USA, 105, 3968-3973. https://doi.org/10.1073/pnas.0710525105
Molognoni, D., Bosch-Jimenez, P., Suarez, J., Pirriera, M.D., & Borràs, E. (2021). How to balance the voltage in serially stacked bioelectrochemical systems. Journal of Power Sources, 491, 229576. https://doi.org/10.1016/j.jpowsour.2021.229576
Myers, C.R., & Myers J.M. (1994). Ferric iron reduction-linked growth yields of Shewanella putrefaciens MR-1. J Appl Bacteriol, 76, 253-258. https://doi.org/10.1111/j.1365-2672.1994.tb01624.x
Okamoto, A., Hashimoto, K., & Nakamura, R. (2012). Long- range electronconduction of Shewanella biofilms mediated by outermembrane C-type cytochromes. Bioelectrochemistry, 85, 61, 65. https://doi.org/10.1016/j.bioelechem.2011.12.003
Ozdemir, M., Enisoglu-Atalay, V., Bermek, H., Ozilhan, S., Tarhan, N., & Catal, T. 2019. Removal of a cannabis metabolite from human urine in microbial fuel cells generating electricity. Bioresource Technology Reports, 5, 121-126. https://doi.org/10.1016/j.biteb.2019.01.003
Park, S. G., Rhee, C., Jadhav, D. A., Eisa, T., Al-Mayyahi, R. B., Shin, S. G., Abdelkareem, M. A., & Chae, K. J. (2023). Tailoring a highly conductive and super-hydrophilic electrode for biocatalytic performance of microbial electrolysis cells. The Science of the total environment, 856(Pt 1), 159105. https://doi.org/10.1016/j.scitotenv.2022.159105
Pasupuleti, S. B., Srikanth, S., Venkata Mohan, S., & Pant, D. (2015). Development of exoelectrogenic bioanode and study on feasibility of hydrogen production using abiotic VITO-CoRE™ and VITO-CASE™ electrodes in a single chamber microbial electrolysis cell (MEC) at low current densities. Bioresource technology, 195, 131–138. https://doi.org/10.1016/j.biortech.2015.06.145
Pozo, G., Lu, Y., Pongy, S., Keller, J., Ledezma, P., & Freguia, S. (2017). Selective cathodic microbial biofilm retention allows a high current-to-sulfide efficiency in sulfate-reducing microbial electrolysis cells. Bioelectrochemistry (Amsterdam, Netherlands), 118, 62–69. https://doi.org/10.1016/j.bioelechem.2017.07.001
Puig, S., Serra, M., Coma, M., Cabré, M., Balaguer, M. D., & Colprim, J. (2010). Effect of pH on nutrient dynamics and electricity production using microbial fuel cells. Bioresource technology, 101(24), 9594–9599. https://doi.org/10.1016/j.biortech.2010.07.082
Pokkuluri, P.R., Londer, Y.Y., Duke, N.E., Pessanha, M., Yang, X., Orshonsky V., Orshonsky, L., Erickson, J., Zagyanskiy, Y., Salgueiro, C.A., & Schiffer, M. (2011). Structure of a novel dodecaheme cytochrome c from Geobacter sulfurreducens reveals an extended 12 nm protein with interacting hemes. J Struct Biol, 174, 223-33. https://doi.org/10.1016/j.jsb.2010.11.022
Quashie, F. K., Feng, K., Fang, A., Agorinya, S., Antwi, P., Kabutey, F. T., & Xing, D. (2021). Efficiency and key functional genera responsible for simultaneous methanation and bioelectricity generation within a continuous stirred microbial electrolysis cell (CSMEC) treating food waste. The Science of the total environment, 757, 143746. https://doi.org/10.1016/j.scitotenv.2020.143746
Rabaey, K., Boon N., Höfte M., & Verstraete W. (2005). Microbial phenazine production enhances electron transfer in biofuel cells. Environ Sci Technol, 39, 3401-3408. https://doi.org/10.1021/es048563o
Rajmohan, P., & Logan, B. E. (2015). Microbial electrolysis cells: A review on recent advances and future prospects. Bioresource Technology, 180, 65-74.
Ren, L., Tokash, J.C., Regan, J.M., & Logan, B.E. (2012). Current generation in microbial electrolysis cells with addition of amorphous ferric hydroxide, Tween 80, or DNA. Int J Hydro Energy, 37, 16943-16950. https://doi.org/10.1016/j.ijhydene.2012.08.119
Rollefson, J.B., Stephen, C.S., Tien, M., & Bond, D.R. (2011). Identification of an extracellular polysaccharide network essential for cytochrome anchoring and biofilm formation in Geobacter sulfurreducens. J Bact, 193, 1023-1233. https://doi.org/10.1128/JB.01092-10
Rossi, R., Baek, G., & Logan, B. E. (2022). Vapor-Fed Cathode Microbial Electrolysis Cells with Closely Spaced Electrodes Enables Greatly Improved Performance. Environmental science & technology, 56(2), 1211–1220. https://doi.org/10.1021/acs.est.1c06769
Satinover, S. J., Rodriguez, M., Jr, Campa, M. F., Hazen, T. C., & Borole, A. P. (2020). Performance and community structure dynamics of microbial electrolysis cells operated on multiple complex feedstocks. Biotechnology for biofuels, 13, 169. https://doi.org/10.1186/s13068-020-01803-y
Sharma, A., Mehdi, S. E. H., Pandit, S., Eun-Oh, S., & Natarajan, V. (2024). Factors affecting hydrogen production in microbial electrolysis cell (MEC): A review. International Journal of Hydrogen Energy, 61, 1473-1484. https://doi.org/10.1016/j.ijhydene.2024.02.193
Singh, N. K., Mathuriya, A. S., Mehrotra, S., Pandit, S., Singh, A., & Jadhav, D. (2024). Advances in bioelectrochemical systems for bio-products recovery. Environmental technology, 45(19), 3853–3876. https://doi.org/10.1080/09593330.2023.2234676
Song, Y. H., Hidayat, S., Kim, H. K., & Park, J. Y. (2016). Hydrogen production in microbial reverse-electrodialysis electrolysis cells using a substrate without buffer solution. Bioresource technology, 210, 56–60. https://doi.org/10.1016/j.biortech.2016.02.021
Sonmez, E., Avci, B., Mohamed, N., & Bermek, H. (2024). Investigation of performance losses in microbial fuel cells with low platinum loadings on air-cathodes. The European Chemistry and Biotechnology Journal, 1, 11–26. https://doi.org/10.62063/ecb-14
Spiess, S., Kucera, J., Seelajaroen, H., Sasiain, A., Thallner, S., Kremser, K., Novak, D., Guebitz, G. M., & Haberbauer, M. (2021). Impact of Carbon Felt Electrode Pretreatment on Anodic Biofilm Composition in Microbial Electrolysis Cells. Biosensors, 11(6), 170. https://doi.org/10.3390/bios11060170
Spiess, S., Sasiain Conde, A., Kucera, J., Novak, D., Thallner, S., Kieberger, N., Guebitz, G. M., & Haberbauer, M. (2022). Bioelectrochemical methanation by utilization of steel mill off-gas in a two-chamber microbial electrolysis cell. Frontiers in bioengineering and biotechnology, 10, 972653. https://doi.org/10.3389/fbioe.2022.972653
Spiess, S., Kucera, J., Vaculovic, T., Birklbauer, L., Habermaier, C., Conde, A. S., Mandl, M., & Haberbauer, M. (2023). Zinc recovery from bioleachate using a microbial electrolysis cell and comparison with selective precipitation. Frontiers in microbiology, 14, 1238853. https://doi.org/10.3389/fmicb.2023.1238853
Sukkasem, C. (2024). Exploring biofilm-forming bacteria for integration into BioCircuit wastewater treatment. The European Chemistry and Biotechnology Journal, 2, 39–52. https://doi.org/10.62063/ecb-28
Ucar, D., Zhang, Y., & Angelidaki, I. (2017). An Overview of Electron Acceptors in Microbial Fuel Cells. Frontiers in microbiology, 8, 643. https://doi.org/10.3389/fmicb.2017.00643
Umar, M. F., Rafatullah, M., Abbas, S. Z., Mohamad Ibrahim, M. N., & Ismail, N. (2021). Advancement in Benthic Microbial Fuel Cells toward Sustainable Bioremediation and Renewable Energy Production. International journal of environmental research and public health, 18(7), 3811. https://doi.org/10.3390/ijerph18073811
Wang, L., Long, F., Liang, D., Xiao, X., & Liu, H. (2021a). Hydrogen production from lignocellulosic hydrolysate in an up-scaled microbial electrolysis cell with stacked bio-electrodes. Bioresource technology, 320(Pt A), 124314. https://doi.org/10.1016/j.biortech.2020.124314
Wang, Y., Xi, B., Jia, X., Li, M., Qi, X., Xu, P., Zhao, Y., Ye, M., & Hao, Y. (2021b). Characterization of hydrogen production and microbial community shifts in microbial electrolysis cells with L-cysteine. The Science of the total environment, 760, 143353. https://doi.org/10.1016/j.scitotenv.2020.143353
Wang, X. T., Zhang, Y. F., Wang, B., Wang, S., Xing, X., Xu, X. J., Liu, W. Z., Ren, N. Q., Lee, D. J., & Chen, C. (2022). Enhancement of methane production from waste activated sludge using hybrid microbial electrolysis cells-anaerobic digestion (MEC-AD) process – A review. Bioresource technology, 346, 126641. https://doi.org/10.1016/j.biortech.2021.126641
Wang, L., & Liu, H. (2024). Microbial electrosynthesis of single cell protein and methane by coupling fast-growing Methanococcus maripaludis with microbial electrolysis cells. Bioresource technology, 393, 130025. https://doi.org/10.1016/j.biortech.2023.130025
Wu, M., Yang, F., Hu, J., Yu, Z., Yu, J., & Chen, J. (2024). Unveiling microbial community structure and metabolic pathway over carbon cloth-titanium nitride-polyaniline biocathode for effective dichloromethane transformation. Environmental pollution (Barking, Essex: 1987), 358, 124486. https://doi.org/10.1016/j.envpol.2024.124486
Xiao, L., Wen, Z., Ci, S., Chen, J., He, Z. (2012). Carbon/iron-based nanorod catalysts for hydrogen production in microbial electrolysis cells. Nano Energy, 1, 751-756. https://doi.org/10.1016/j.nanoen.2012.06.002
Xue, W., Zhou, Q., & Li, F. (2019). Bacterial community changes and antibiotic resistance gene quantification in microbial electrolysis cells during long-term sulfamethoxazole treatment. Bioresource technology, 294, 122170. https://doi.org/10.1016/j.biortech.2019.122170
Yang, Y., Xu, M., Guo, J., Sun, G. (2012). Bacterial extracellular electron transfer in bioelectrochemical systems. Process Biochem, https://doi.org/10.1016/j.procbio.2012.07.032.
Yu, Z., Leng, X., Zhao, S., Ji, J., Zhou, T., Khan, A., Kakde, A., Liu, P., & Li, X. (2018). A review on the applications of microbial electrolysis cells in anaerobic digestion. Bioresource technology, 255, 340–348. https://doi.org/10.1016/j.biortech.2018.02.003
Yu, H., Huang, L., Zhang, G., & Zhou, P. (2022). Physiological metabolism of electrochemically active bacteria directed by combined acetate and Cd(II) in single-chamber microbial electrolysis cells. Journal of hazardous materials, 424(Pt C), 127538. https://doi.org/10.1016/j.jhazmat.2021.127538
Yuan, H., & He, Z. (2017). Platinum Group Metal-free Catalysts for Hydrogen Evolution Reaction in Microbial Electrolysis Cells. Chemical record (New York, N.Y.), 17(7), 641–652. https://doi.org/10.1002/tcr.201700007
Zhang, Y., Merrill, M.D., & Logan, B.E. (2010). The use and optimization of stainess steel mesh cathodes in microbial electrolysis cells. Int J Hydro En, 35, 12020-12028. https://doi.org/10.1016/j.ijhydene.2010.08.064
Zhang, E. R., Liu, L., & Cui, Y. Y. (2012). Effect of PH on the Performance of the Anode in Microbial Fuel Cells. Advanced Materials Research, 608–609, 884–888. https://doi.org/10.4028/www.scientific.net/amr.608-609.884
Zhang, Y., & Angelidaki, I. (2014). Microbial electrolysis cells turning to be versatile technology: recent advances and future challenges. Water research, 56, 11–25. https://doi.org/10.1016/j.watres.2014.02.031
Zhang, W., Liu, M., Gu, X., Shi, Y., Deng, Z., & Cai, N. (2023). Water Electrolysis toward Elevated Temperature: Advances, Challenges and Frontiers. Chemical reviews, 123(11), 7119–7192. https://doi.org/10.1021/acs.chemrev.2c00573
Zheng, T., Li, J., Ji, Y., Zhang, W., Fang, Y., Xin, F., Dong, W., Wei, P., Ma, J., & Jiang, M. (2020). Progress and Prospects of Bioelectrochemical Systems: Electron Transfer and Its Applications in the Microbial Metabolism. Frontiers in bioengineering and biotechnology, 8, 10. https://doi.org/10.3389/fbioe.2020.00010
Zhou, M., Yang, J., Wang, H., Jin, T., Xu, D., & Gu, T. (2013). Microbial fuel cells and microbial electrolysis cells for the production of bioelectricity and biomaterials. Environmental technology, 34(13-16), 1915–1928. https://doi.org/10.1080/09593330.2013.813951
Zhou, R., Zhou, S., & He, C. (2020). Quantitative evaluation of effects of different cathode materials on performance in Cd(II)-reduced microbial electrolysis cells. Bioresource technology, 307, 123198. https://doi.org/10.1016/j.biortech.2020.123198
Zhu, Y., Zhang, Y., Wang, X., & Li, Y. (2021). Optimization of Hydrogen Production in Microbial Electrolysis Cells: A Review. Energy & Fuels, 35, 10509-10524.
Zhu, Y., Guo, M., Qi, X., Li, M., Guo, M., & Jia, X. (2024). Enhanced degradation and methane production of food waste anaerobic digestate using an integrated system of anaerobic digestion and microbial electrolysis cells for long-term operation. Environmental science and pollution research international, 31(27), 39637–39649. https://doi.org/10.1007/s11356-024-33525-1